3E63B32F1D3E6408C9DE45F8763064E2E21170A93269BCEE890C9270549BB522F48EBDE0E7621B136FA777633ABFDEA0

Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2-ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA (By similarity).

Source:http://purl.uniprot.org/SHA-384/3E63B32F1D3E6408C9DE45F8763064E2E21170A93269BCEE890C9270549BB522F48EBDE0E7621B136FA777633ABFDEA0