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Biochem. Biophys. Res. Commun.

Human T lymphoblastoid cell line CEM-ON belongs to a helper/inducer subclass and secretes M-CSF into medium constitutively. We have isolated a full-length cDNA clone for this factor from a cDNA library of this cell line. The cDNA was 2.5 kb and coded for a 554 amino acid polypeptide precursor including signal sequence. The Northern blot analysis showed that the major transcript of M-CSF is about 4.2 kb. We have studied the expression of not only the wild type plasmid, but also the five C-terminal deletion mutants encoding N-terminal 154, 163, 170, 177, and 185 amino acid residues in monkey COS-1 cells. The results showed that at least C-terminal 377 amino acid residues of human M-CSF are not essential to manifest the in vitro biological activity on murine bone marrow cells.

Source:http://purl.uniprot.org/citations/2660794

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http://purl.uniprot.org/cit...rdfs:commentHuman T lymphoblastoid cell line CEM-ON belongs to a helper/inducer subclass and secretes M-CSF into medium constitutively. We have isolated a full-length cDNA clone for this factor from a cDNA library of this cell line. The cDNA was 2.5 kb and coded for a 554 amino acid polypeptide precursor including signal sequence. The Northern blot analysis showed that the major transcript of M-CSF is about 4.2 kb. We have studied the expression of not only the wild type plasmid, but also the five C-terminal deletion mutants encoding N-terminal 154, 163, 170, 177, and 185 amino acid residues in monkey COS-1 cells. The results showed that at least C-terminal 377 amino acid residues of human M-CSF are not essential to manifest the in vitro biological activity on murine bone marrow cells.lld:uniprot
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http://purl.uniprot.org/cit...uniprot:nameBiochem. Biophys. Res. Commun.lld:uniprot
http://purl.uniprot.org/cit...uniprot:authorTakahashi M.lld:uniprot
http://purl.uniprot.org/cit...uniprot:authorHirai Y.lld:uniprot
http://purl.uniprot.org/cit...uniprot:authorNishida T.lld:uniprot
http://purl.uniprot.org/cit...uniprot:authorNakai S.lld:uniprot
http://purl.uniprot.org/cit...uniprot:authorTakano M.lld:uniprot
http://purl.uniprot.org/cit...uniprot:authorHirato T.lld:uniprot
http://purl.uniprot.org/cit...uniprot:authorNagamura K.lld:uniprot
http://purl.uniprot.org/cit...uniprot:authorKamogashira T.lld:uniprot
http://purl.uniprot.org/cit...uniprot:date1989lld:uniprot
http://purl.uniprot.org/cit...uniprot:pages892-901lld:uniprot
http://purl.uniprot.org/cit...uniprot:titleAmino-terminal region of human macrophage colony-stimulating factor (M-CSF) is sufficient for its in vitro biological activity: molecular cloning and expression of carboxyl-terminal deletion mutants of human M-CSF.lld:uniprot
http://purl.uniprot.org/cit...uniprot:volume161lld:uniprot
http://purl.uniprot.org/cit...dc-term:identifierdoi:10.1016/0006-291X(89)92683-1lld:uniprot
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