The Israeli isolate of Tomato yellow leaf curl geminivirus (TYLCV-Is) is a major tomato pathogen, causing extensive crop losses both in the New and Old World. Surprisingly, however, little is known about the molecular mechanisms of TYLCV-Is interactions with tomato cells. Here, we have identified a TYLCV-Is protein, V2, which acts as a suppressor of RNA silencing and which is unrelated to presently known viral suppressors. Specifically, V2, but not other proteins of TYLCV-Is, inhibited RNA silencing of a reporter transgene, GFP. This inhibition elevated the cellular levels of the GFP transcript and the GFP protein, but it had no apparent effect on the accumulation of GFP-specific short interfering RNAs (siRNAs), suggesting that TYLCV-Is V2 targets a step in the RNA silencing pathway which is subsequent to the Dicer-mediated cleavage of dsRNA. Visualization of the sub-cellular localization of TYLCV-Is V2 in plant protoplasts and tissues showed that this protein is associated with cytoplasmic strands and inclusion bodies in the cortical regions of the cell.
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http://purl.uniprot.org/cit... | rdfs:comment | The Israeli isolate of Tomato yellow leaf curl geminivirus (TYLCV-Is) is a major tomato pathogen, causing extensive crop losses both in the New and Old World. Surprisingly, however, little is known about the molecular mechanisms of TYLCV-Is interactions with tomato cells. Here, we have identified a TYLCV-Is protein, V2, which acts as a suppressor of RNA silencing and which is unrelated to presently known viral suppressors. Specifically, V2, but not other proteins of TYLCV-Is, inhibited RNA silencing of a reporter transgene, GFP. This inhibition elevated the cellular levels of the GFP transcript and the GFP protein, but it had no apparent effect on the accumulation of GFP-specific short interfering RNAs (siRNAs), suggesting that TYLCV-Is V2 targets a step in the RNA silencing pathway which is subsequent to the Dicer-mediated cleavage of dsRNA. Visualization of the sub-cellular localization of TYLCV-Is V2 in plant protoplasts and tissues showed that this protein is associated with cytoplasmic strands and inclusion bodies in the cortical regions of the cell. | lld:uniprot |
http://purl.uniprot.org/cit... | skos:exactMatch | http://purl.uniprot.org/pub... | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:name | Virology | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Citovsky V. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Arazi T. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Gafni Y. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Levy Y. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Glick E. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:author | Zrachya A. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:date | 2007 | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:pages | 159-165 | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:title | Suppressor of RNA silencing encoded by Tomato yellow leaf curl virus-Israel. | lld:uniprot |
http://purl.uniprot.org/cit... | uniprot:volume | 358 | lld:uniprot |
http://purl.uniprot.org/cit... | dc-term:identifier | doi:10.1016/j.virol.2006.08.016 | lld:uniprot |
uniprot-protein:P27269 | uniprot:citation | http://purl.uniprot.org/cit... | lld:uniprot |
http://linkedlifedata.com/r... | uniprot:source | http://purl.uniprot.org/cit... | lld:uniprot |
http://linkedlifedata.com/r... | rdf:object | http://purl.uniprot.org/cit... | lld:uniprot |