. "Reviewed: D'Eustachio, P, 2007-04-30 14:43:26"^^ . "Authored: Jassal, B, 2007-01-19 10:34:59"^^ . "Edited: D'Eustachio, P, 2007-02-17 19:40:28"^^ . "3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-27-al, NADPH + H+, and O2 react to form 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoate (THCA), NADP+, and H2O. This oxidation reaction occurs in the mitochondrial matrix, catalyzed by CYP27A1."^^ . . . . . . . "3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-27-al is oxidized to 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoate (THCA)"^^ . "3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-27-al + NADPH + H+ + O2 => 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoate (THCA) + NADP+ + H2O"^^ . . . . . . . .