. "Reviewed: Jassal, B, 2009-02-27 15:21:18"^^ . "Edited: D'Eustachio, P, 2009-03-18 13:33:40"^^ . "Authored: D'Eustachio, P, 2009-03-16 18:50:14"^^ . "Peroxisomal HSD17B4 dimer catalyzes the reaction of 3-hydroxyhexacosanoyl-CoA and NAD+ to form 3-ketohexacosanoyl-CoA and NADH + H+. The enzyme is bifunctional - an aminoterminal domain catalyzes the dehydrogenation of a variety of 3-hydroxyacyl-CoA's, the reaction annotated here, and a carboxyterminal domain catalyzes the hydration of a variety of trans-2,3-dehydroacyl-CoA's (Jiang et al. 1996, 1997). Defects in the enzyme are associated with a severe disorder of peroxisomal fatty acid metabolism in humans (Ferdinandusse et al. 2006)."^^ . . . . . . . . "3-hydroxyhexacosanoyl-CoA + NAD+ => 3-ketohexacosanoyl-CoA + NADH + H+"^^ . "1.1.1.35"^^ . . . . . .