. "Catalysis of the transfer of a methyl group from 5,10-methylenetetrahydrofolate to the C5 atom of the uridine residue at position 54 in a tRNA molecule. This occurs in most Gram-positive bacteria and some Gram-negative bacteria." . . . "RUMT" . "5,10-methylenetetrahydrofolate-dependent tRNA (m5U54) methyltransferase activity" . .