. "Catalysis of the reaction: S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing thymine at position U54 of a transfer RNA. This occurs in most Gram-negative bacteria, some archae, and eukaryotes." . . . . "RUMT" . "S-adenosylmethionine-dependent tRNA (m5U54) methyltransferase activity" . "M5U-methyltransferase activity" . "ribothymidyl synthase activity" . "RUMT activity" . "S-adenosyl methionine-dependent tRNA (m5U54) methyltransferase activity" . "S-adenosyl-L-methionine:tRNA (uracil-5-)-methyltransferase activity" . "transfer RNA uracil 5-methyltransferase activity" . "transfer RNA uracil methylase activity" . "tRNA (uracil-5-)-methyltransferase activity" . "tRNA uracil 5-methyltransferase activity" . "tRNA:m(5)U54-methyltransferase activity" . "tRNA:m5U54-methyltransferase activity" . . .