. "The bacterial enzyme modifies the wobble base of the CAU anticodon of tRNA(Ile) at the oxo group in position 2 of cytidine(34)." . "This modification determines both codon and amino acid specificities of tRNA(Ile)." . . . "tRNA(Ile)-lysidine synthetase" . "Isoleucine-specific transfer ribonucleate lysidine synthetase" . "(tRNA(Ile2))-cytidine(34) + L-lysine + ATP = (tRNA(Ile2))-lysidine(34) + AMP + diphosphate + H(2)O." .