. "The enzyme acetylates the wobble base C34 of the CAU anticodon of elongation-specific tRNA(Met)." . "Escherichia coli TmcA strictly discriminates elongator tRNA(Met) from tRNA(Ile), which is structurally similar and has the same anticodon loop, mainly by recognizing the C27-G43 pair in the anticodon stem." . "The enzyme can use GTP in place of ATP for formation of N(4)-acetylcytidine." . . . "tRNA(Met) cytidine acetyltransferase" . "(Elongator tRNA(Met))-cytidine(34) + ATP + acetyl-CoA = (elongator tRNA(Met))-N(4)-acetylcytidine(34) + ADP + phosphate + CoA." .