. "The 465,813 base pair sequence corresponding to the 40.1-50.0 min region on the genetic map of Escherichia coli K-12 (W3110) was determined. Analysis of the sequence revealed that this region contained at least 466 potential open reading frames, of which 187 (40%) were previously reported, 105 (23%) were homologous to other known genes, 103 (22%) were identical or similar to hypothetical genes registered in databases, and the remaining 71 (15%) did not show a significant similarity to any other gene. At the 45.2-46.0 min region, we found a very large cluster of about 30 genes, whose functions are involved in the biosynthesis of polysaccharides as the components of outer membranes. In addition, we identified a new asn-tRNA gene, designated asnW, between the asnT and asnU genes and a new lysogenic phage attachment site as the cis-element." . . . "DNA Res." . "Itoh T." . "Miki T." . "Isono K." . "Hayashi K." . "Fujita K." . "Kimura S." . "Takeda J." . "Nakamura Y." . "Yamamoto Y." . "Aiba H." . "Mori T." . "Horiuchi T." . "Mori H." . "Baba T." . "Tagami H." . "Kitagawa M." . "Oshima T." . "Nishio Y." . "Kitakawa M." . "Nashimoto H." . "Mizobuchi K." . "Makino K." . "Wada C." . "Kasai H." . "Saito N." . "Takemoto K." . "Inada T." . "Sampei G." . "Seki Y." . "Nakade S." . "Motomura K." . "Sivasundaram S." . "true"^^ . "1996"^^ . "379-392" . "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." . "3" . "doi:10.1093/dnares/3.6.379" .