. "Rab proteins are small GTPases involved in the regulation of intracellular membrane traffic in mammalian cells. In order to find Rab-interacting proteins we performed a two-hybrid screening using a human brain cDNA library. Here we report the isolation of a full-length human cDNA clone coding for a protein of 185 amino acids. This protein interacts strongly with the Rab4b, Rab5a, and Rab5c proteins and weakly with Rab4a, Rab6, Rab7, Rab17, and Rab22 in the two-hybrid assay. Comparison with the Data Bank revealed that this clone represents the human homolog of the previously isolated rat Prenylated Rab Acceptor (rPRA1). Analysis of mRNA expression shows a single abundant mRNA of about 0.8 kb ubiquitously expressed. Western blot analysis of the overexpressed protein shows a band of the expected size equally distributed between cytosol and membranes." . . . "Biochem. Biophys. Res. Commun." . "Bruni C.B." . "Bucci C." . "Chiariello M." . "Lattero D." . "Maiorano M." . "1999"^^ . "657-662" . "Interaction cloning and characterization of the cDNA encoding the human prenylated rab acceptor (PRA1)." . "258" . "doi:10.1006/bbrc.1999.0651" .