. "Genetically modified source" . . "Entamoeba histolytica HM-1:IMSS" . . "Escherichia coli" . "AVA0421" . "EHI_153650" . "Expressed with an N-terminal his tag and 3C protease cleavage site. Expression tag not removed prior to crystallization. Construct contains an Ala at residue 85 not a Val as indicated by genomics project. Residue 85 is in the interior of the protein and does not appear to accommodate a Val. Switching from a Val to Ala is GYN." .