. . . . . . . . . . "Protein kinase C phosphorylates HIV-1 Nef at threonine 15" . "Treatment of cells with protein kinase C (PKC) inhibitors reduces HIV-1 Nef phosphorylation in vivo, and phosphorylation of Nef increases 8- to 12-fold after treatment with phorbol myristate acetate; these data indicate PKC phosphorylates Nef" . "PKC activity and serine 6 in HIV-1 Nef are required for Nef-supported transcription, infectivity, and replication" . "requires" . "phosphorylated by" .