. . . . . . . . . . . . . "ISG15 inhibits budding of HIV-1 by disrupting the association between Vps4 and the Gag budding complex" . "Replacing the NC-p1-p6 region of HIV-1 Gag with the leucine zipper domain of yeast GCN4 overcomes inhibition of HIV-1 particle budding by dominant-negative ESCRT proteins such as CHMP3 and Vps4A" . "VPS4A increases co-localization of HIV-1 Gag with the ESCRT proteins Chmp1b, Chmp4b, and Chmp4c at sites of HIV-1 assembly" . "VPS4A is co-localized with nascent HIV-1 Gag molecules at the host cell plasma membrane by using live-cell imaging" . "The budding defect of p6-deficient HIV-1 Gag is caused primarily by C-terminal exposure of p1, which activates a cis-acting inhibitory budding signal (IBS) and impairs an exosomes and microvesicles (EMV) cargo-VPS4 interaction" . "Affinity Capture-Western" . "BioGRID" . "inhibits" . "inhibited by" . "co-localizes with" . "associates with" . "recruited by" . .