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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1999-2-16
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pubmed:abstractText |
T cell activation is controlled by the coordination of stimulatory and negative regulatory signals which are not completely defined. In this study we tested for a possible direct effect of CD14 on the regulation of T cell activation and function. We show that soluble CD14 (sCD14) induces inhibition of antigen-mediated peripheral blood mononuclear cells (PBMC) proliferation and anti-CD3-mediated proliferation of CD4+CD8+, CD4+CD8+ and CD4+CD8+ Tcell clones. This effect is not due to cell death, but results from a marked inhibition of IL-2 production. Proliferation of T cell clones due to exogenous IL-2 is not affected by sCD14. We also found that sCD14 inhibits production of another Th1-like cytokine, IFN-gamma and a Th2-like cytokine, IL-4. Importantly, sCD14 induces a progressive accumulation of the inhibitory protein IkappaB-alpha. We show that sCD14 binds to activated T cells. Following cell activation, biotinylated sCD14 stains CD3+ PBMC, as well as human T cell clones with varying intensity. The binding is saturable, can be inhibited by excess of unlabeled sCD14 and, following binding, sCD14 is internalized. Collectively, these findings reveal a previously unrecognized function of sCD14, namely its capacity to negatively regulate T lymphocyte activation and function by interacting directly with activated T cells.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD14,
http://linkedlifedata.com/resource/pubmed/chemical/Biotin,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/I-kappa B Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappaB inhibitor alpha,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-2
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0014-2980
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
29
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
265-76
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pubmed:dateRevised |
2010-8-25
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pubmed:meshHeading |
pubmed-meshheading:9933108-Antigens, CD14,
pubmed-meshheading:9933108-Biological Transport, Active,
pubmed-meshheading:9933108-Biotin,
pubmed-meshheading:9933108-Cell Division,
pubmed-meshheading:9933108-Cell Survival,
pubmed-meshheading:9933108-Clone Cells,
pubmed-meshheading:9933108-DNA-Binding Proteins,
pubmed-meshheading:9933108-Humans,
pubmed-meshheading:9933108-I-kappa B Proteins,
pubmed-meshheading:9933108-Interferon-gamma,
pubmed-meshheading:9933108-Interleukin-2,
pubmed-meshheading:9933108-Interleukin-4,
pubmed-meshheading:9933108-Jurkat Cells,
pubmed-meshheading:9933108-Kinetics,
pubmed-meshheading:9933108-Lymphocyte Activation,
pubmed-meshheading:9933108-Receptors, Interleukin-2,
pubmed-meshheading:9933108-Solubility,
pubmed-meshheading:9933108-T-Lymphocytes
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pubmed:year |
1999
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pubmed:articleTitle |
Soluble CD14 acts as a negative regulator of human T cell activation and function.
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pubmed:affiliation |
Department of Medicine, University of Wales, College of Medicine, Cardiff, GB.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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