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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1998-12-21
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pubmed:abstractText |
The activity of hepatic carnitine palmitoyltransferase I (CPT-I) may be modulated by interactions with cytoskeletal components [Velasco et al. (1998) J. Biol. Chem. 273, 21497-21504]. We have studied whether the AMP-activated protein kinase (AMPK) is involved in this process. AMPK stimulated CPT-I in permeabilized hepatocytes but not in isolated liver mitochondria. In addition, AMPK abrogated the inhibition of CPT-I of isolated mitochondria induced by a cytoskeletal fraction. These two effects of AMPK were not evident when the kinase was inactivated by pretreatment with protein phosphatase 2C. Cytokeratins 8 and 18 were phosphorylated by AMPK in vitro and by incubation of intact hepatocytes with 5-aminoimidazole-4-carboxamide ribonucleoside, a cell-permeable activator of AMPK. These results provide the first evidence that AMPK stimulates CPT-I by direct phosphorylation of cytoskeletal components.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
20
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pubmed:volume |
439
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
317-20
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:9845345-AMP-Activated Protein Kinases,
pubmed-meshheading:9845345-Aminoimidazole Carboxamide,
pubmed-meshheading:9845345-Animals,
pubmed-meshheading:9845345-Carnitine O-Palmitoyltransferase,
pubmed-meshheading:9845345-Cell Membrane Permeability,
pubmed-meshheading:9845345-Cells, Cultured,
pubmed-meshheading:9845345-Cytoskeleton,
pubmed-meshheading:9845345-Enzyme Activation,
pubmed-meshheading:9845345-Keratins,
pubmed-meshheading:9845345-Liver,
pubmed-meshheading:9845345-Malonyl Coenzyme A,
pubmed-meshheading:9845345-Mitochondria, Liver,
pubmed-meshheading:9845345-Multienzyme Complexes,
pubmed-meshheading:9845345-Phosphorylation,
pubmed-meshheading:9845345-Protein Kinases,
pubmed-meshheading:9845345-Protein-Serine-Threonine Kinases,
pubmed-meshheading:9845345-Rats,
pubmed-meshheading:9845345-Ribonucleotides
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pubmed:year |
1998
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pubmed:articleTitle |
Evidence that the AMP-activated protein kinase stimulates rat liver carnitine palmitoyltransferase I by phosphorylating cytoskeletal components.
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pubmed:affiliation |
Department of Biochemistry and Molecular Biology I, School of Biology, Complutense University, Madrid, Spain.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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