Linked Life Data

9541175

Source:http://linkedlifedata.com/resource/pubmed/id/9541175

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1998-5-26
pubmed:abstractText
An abnormality of the physical properties of the cell membrane may underlie the defect that unites the clinical and biochemical abnormalities found in subjects with diabetic nephropathy. The cell membrane is linked both structurally and functionally with the cytoskeleton. The fluorescence anisotropy, a measure of membrane fluidity, was studied at baseline and after modulation of cytoskeletal proteins by thiol group alkylation with N-ethylmaleimide (NEM). 1,6-diphenyl-1,3,5-hexatriene (DPH) was used to assess anisotropy in the deep hydrophobic regions of the lipid bilayer and trimethylammonium-diphenylhexatriene (TMA-DPH) was used to assess the superficial, relatively hydrophilic regions. We compared 17 subjects with insulin-dependent diabetes mellitus (IDDM) and nephropathy with 17 control subjects with IDDM and 24 non-diabetic control subjects. Median TMA-DPH anisotropy (0.271 (0.239-0.332) vs 0.269 (0.258-0.281) vs 0.275 (0.246-0.287)) and DPH anisotropy (0.221 (0.193-0.261) vs 0.227 (0.197-0.253) vs 0.226 (0.193-0.245)) were similar in erythrocytes from the three groups. However after alkylation of protein thiol groups with NEM clear differences emerged. In the control subjects with and without IDDM there was a significant fall in TMA-DPH anisotropy compared to the subjects with diabetic nephropathy in whom the addition of NEM had no effect (deltaTMA-DPH anisotropy -0.005 (-0.020 - +0.006) vs -0.005 (-0.011 - +0.016) vs +0.002 (-0.010 - +0.008) p < 0.001). This finding was confirmed when the deep regions of the lipid bilayer were assessed using DPH (deltaDPH anisotropy -0.017 (-0.029 - -0.007.) vs -0.015 (-0.029 - +0.001) vs + 0.003 (-0.021 - +0.018) p < 0.001). We conclude that cytoskeletal modulation of the physical properties of the cell membrane lipids by proteins is abnormal in subjects with diabetic nephropathy. Such an abnormality could explain some of the clinical and metabolic abnormalities found in this condition.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0012-186X
pubmed:author
pubmed:issnType
Print
pubmed:volume
41
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
337-42
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:9541175-Adult, pubmed-meshheading:9541175-Angiotensin-Converting Enzyme Inhibitors, pubmed-meshheading:9541175-Case-Control Studies, pubmed-meshheading:9541175-Creatinine, pubmed-meshheading:9541175-Diabetes Mellitus, Type 1, pubmed-meshheading:9541175-Diabetic Nephropathies, pubmed-meshheading:9541175-Diphenylhexatriene, pubmed-meshheading:9541175-Erythrocyte Membrane, pubmed-meshheading:9541175-Ethylmaleimide, pubmed-meshheading:9541175-Female, pubmed-meshheading:9541175-Fluorescence Polarization, pubmed-meshheading:9541175-Fluorescent Dyes, pubmed-meshheading:9541175-Hemoglobin A, Glycosylated, pubmed-meshheading:9541175-Humans, pubmed-meshheading:9541175-Male, pubmed-meshheading:9541175-Membrane Fluidity, pubmed-meshheading:9541175-Middle Aged, pubmed-meshheading:9541175-Sulfhydryl Reagents, pubmed-meshheading:9541175-Triglycerides
pubmed:year
1998
pubmed:articleTitle
Abnormal regulation of cell membrane fluidity in diabetic nephropathy.
pubmed:affiliation
Department of Medicine, University of Newcastle upon Tyne, UK.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't