Linked Life Data

9525590

Source:http://linkedlifedata.com/resource/pubmed/id/9525590

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1998-4-17
pubmed:abstractText
The interaction of the human immunodeficiency virus type 1 (HIV-1) Pr55Gag molecule with the plasma membrane of an infected cell is an essential step of the viral life cycle. Myristic acid and positively charged residues within the N-terminal portion of MA constitute the membrane-binding domain of Pr55Gag. A separate assembly domain, termed the interaction (I) domain, is located nearer the C-terminal end of the molecule. The I domain is required for production of dense retroviral particles, but has not previously been described to influence the efficiency of membrane binding or the subcellular distribution of Gag. This study used a series of Gag-green fluorescent protein fusion constructs to define a region outside of MA which determines efficient plasma membrane interaction. This function was mapped to the nucleocapsid (NC) region of Gag. The minimal region in a series of C-terminally truncated Gag proteins conferring plasma membrane fluorescence was identified as the N-terminal 14 amino acids of NC. This same region was sufficient to create a density shift in released retrovirus-like particles from 1.13 to 1.17 g/ml. The functional assembly domain previously termed the I domain is thus required for the efficient plasma membrane binding of Gag, in addition to its role in determining the density of released particles. We propose a model in which the I domain facilitates the interaction of the N-terminal membrane-binding domain of Pr55Gag with the plasma membrane.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-1469349, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-1501299, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-1548743, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-1631144, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-1652977, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-1710290, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-1883539, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-2168981, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-2234068, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-2458920, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-2926863, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-3141927, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-3643678, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-7500330, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-7509280, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-7524664, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-7585960, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-7666546, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-7666550, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-7685391, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-7745687, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-7853517, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-7859280, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-7918387, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-7966591, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-8035530, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-8035531, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-8105392, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-8139035, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-8151785, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-8156990, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-8183954, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-8230441, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-8289249, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-8331736, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-8371356, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-8386872, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-8411340, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-8411352, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-8510214, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-8551560, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-8551627, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-8642704, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-8764091, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-8794295, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-8970978, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-9151833, http://linkedlifedata.com/resource/pubmed/commentcorrection/9525590-9261380
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
72
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2723-32
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
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