Linked Life Data

9388294

Source:http://linkedlifedata.com/resource/pubmed/id/9388294

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1998-1-26
pubmed:abstractText
As part of an effort to identify random carbon-source-regulated promoters in the Saccharomyces cerevisiae genome, we discovered that a mitochondrial DNA fragment is capable of directing glucose-repressible expression of a reporter gene. This fragment (CR24) originated from the mitochondrial genome adjacent to a transcription initiation site. Mutational analyses identified a GC cluster within the fragment that is required for transcriptional induction. Repression of nuclear CR24-driven transcription required Reg1p, indicating that this mitochondrially derived promoter is a member of a large group of glucose-repressible nuclear promoters that are similarly regulated by Reg1p. In vivo and in vitro binding assays indicated the presence of factors, located within the nucleus and the mitochondria, that bind to the GC cluster. One or more of these factors may provide a regulatory link between the nucleus and mitochondria.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0172-8083
pubmed:author
pubmed:issnType
Print
pubmed:volume
32
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
389-98
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:9388294-Base Sequence, pubmed-meshheading:9388294-Cell Nucleus, pubmed-meshheading:9388294-DNA, Fungal, pubmed-meshheading:9388294-DNA, Mitochondrial, pubmed-meshheading:9388294-DNA-Binding Proteins, pubmed-meshheading:9388294-Fungal Proteins, pubmed-meshheading:9388294-Gene Expression Regulation, Fungal, pubmed-meshheading:9388294-Glucose, pubmed-meshheading:9388294-Molecular Sequence Data, pubmed-meshheading:9388294-Mutation, pubmed-meshheading:9388294-Phosphoprotein Phosphatases, pubmed-meshheading:9388294-Promoter Regions, Genetic, pubmed-meshheading:9388294-Protein Phosphatase 1, pubmed-meshheading:9388294-Saccharomyces cerevisiae, pubmed-meshheading:9388294-Saccharomyces cerevisiae Proteins, pubmed-meshheading:9388294-Transcription, Genetic, pubmed-meshheading:9388294-Transcriptional Activation
pubmed:year
1997
pubmed:articleTitle
A Saccharomyces cerevisiae mitochondrial DNA fragment activates Reg1p-dependent glucose-repressible transcription in the nucleus.
pubmed:affiliation
Department of Biological Sciences, SS Box 5018, University of Southern Mississippi, Hattiesburg, Mississippi 39406-5018, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.