Linked Life Data

9361281

Source:http://linkedlifedata.com/resource/pubmed/id/9361281

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5-6
pubmed:dateCreated
1998-1-8
pubmed:abstractText
The developing hindbrain is transiently subdivided into structural repeat units, rhombomeres, whose formation is matched by both differential regulatory gene expression and a metameric pattern of early neuronal differentiation and axogenesis. Individual rhombomeres are polyclonal cell lineage restriction units; once defined by transverse interrhombomere interfaces, cells are confined within the territory of a single rhombomere. In order to assess the relevance of this restriction to hindbrain development. It is necessary to understand the underlying mechanism. One possibility is that cells of adjacent rhombomeres acquire differential affinities or adhesive properties. To explore this possibility, we isolated rhombomere cells, mixed them together in short-term aggregation cultures, and assessed the composition of the resulting aggregates. We found that rhombomeres do differ in their affinity: cells from even-numbered rhombomeres sort out from cells of odd-numbered rhombomeres. They also segregate from cells of other even-numbered rhombomeres but to a much lesser extent. This selective cell affinity operates from the time of rhombomere formation until late stages in development. The region-specific segregation was abolished when Ca(2+)-dependent adhesion molecules were inactivated but not when Ca(2+)-independent adhesion molecules were inactivated. These findings suggest that distinct cell affinity restricts cell mixing between adjacent rhombomeres and may be involved in establishing the series of discrete compartments, thereby maintaining anteroposterior positional information during hindbrain development. These results support a general role for cell adhesion molecules in subdividing CNS territories.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1044-7431
pubmed:author
pubmed:issnType
Print
pubmed:volume
9
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
448-59
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Segregation of rhombomeres by differential chemoaffinity.
pubmed:affiliation
Department of Developmental Neurobiology, UMDS, Guy's Hospital, London, England.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't