Linked Life Data

9329616

Source:http://linkedlifedata.com/resource/pubmed/id/9329616

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4B
pubmed:dateCreated
1997-10-29
pubmed:abstractText
A TPS-reactive protein fragment from a human colon adenocarcinoma cell line was purified to electrophoretic homogeneity. N-terminal sequence analysis of the purified 13 kDa protein fragment demonstrated that the component was a fragment of human cytokeratin 18. The M3 monoclonal antibody (detector antibody in the TPS assay) was applied for screening of an expression library. The M3-reactive phage clones were subcloned and PCR amplified. DNA-sequence analysis revealed it to contain a nucleotide fragment corresponding to human cytokeratin 18. Smaller fragment, engineered by PCR and expressed as fusion proteins, demonstrated that the M3 epitope is localized to human cytokeratin 18, amino acid residues 322-340.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0250-7005
pubmed:author
pubmed:issnType
Print
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3121-4
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:articleTitle
Tissue polypeptide specific antigen (TPS) detects a specific epitope structure on human cytokeratin.
pubmed:affiliation
BEKI Diagnostics, AB, Stockholm, Sweden.
pubmed:publicationType
Journal Article