Linked Life Data

8194193

Source:http://linkedlifedata.com/resource/pubmed/id/8194193

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1994-6-27
pubmed:abstractText
Expression of the intermediate filament protein vimentin, and loss of the cellular adhesion protein uvomorulin (E-cadherin) have been associated with increased invasiveness of established human breast cancer cell lines in vitro and in vivo. In the current study, we have further examined these relationships in oncogenically transformed human mammary epithelial cells. A normal human mammary epithelial strain, termed 184, was previously immortalized with benzo[a]pyrene, and two distinct sublines were derived (A1N4 and 184B5). These sublines were infected with retroviral vectors containing a single or two oncogenes of the nuclear, cytoplasmic, and plasma membrane-associated type (v-rasH, v-rasKi, v-mos, SV40T and c-myc). All infectants have been previously shown to exhibit some aspects of phenotypic transformation. In the current study, cellular invasiveness was determined in vitro using Matrigel, a reconstituted basement membrane extract. Lineage-specific differences were observed with respect to low constitutive invasiveness and invasive changes after infection with ras, despite similar ras-induced transformation of each line. Major effects on cellular invasiveness were observed after infection of the cells with two different oncogenes (v-rasH + SV40T and v-rasH + v-mos). In contrast, the effects of single oncogenes were only modest or negligible. All oncogenic infectants demonstrated increased attachment to laminin, but altered secretion of the 72 kDa and 92 kDa gelatinases was not associated with any aspect of malignant progression. Each of the two highly invasive double oncogene transformants were vimentin-positive and uvomorulin-negative, a phenotype indicative of the epithelial-mesenchymal transition (EMT) previously associated with invasiveness of established human breast cancer cell lines. Weakly invasive untransformed mammary epithelial cells in this study were positive for both vimentin and uvomorulin, suggesting that uvomorulin may over-ride the otherwise vimentin-associated invasiveness.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0262-0898
pubmed:author
pubmed:issnType
Print
pubmed:volume
12
pubmed:geneSymbol
mos, myc, ras
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
181-94
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:8194193-Animals, pubmed-meshheading:8194193-Basement Membrane, pubmed-meshheading:8194193-Breast, pubmed-meshheading:8194193-Cadherins, pubmed-meshheading:8194193-Cell Adhesion, pubmed-meshheading:8194193-Cell Transformation, Neoplastic, pubmed-meshheading:8194193-Chemotaxis, pubmed-meshheading:8194193-Collagen, pubmed-meshheading:8194193-Drug Combinations, pubmed-meshheading:8194193-Epithelial Cells, pubmed-meshheading:8194193-Gelatinases, pubmed-meshheading:8194193-Genes, mos, pubmed-meshheading:8194193-Genes, myc, pubmed-meshheading:8194193-Genes, ras, pubmed-meshheading:8194193-Humans, pubmed-meshheading:8194193-Laminin, pubmed-meshheading:8194193-Mice, pubmed-meshheading:8194193-Mice, Nude, pubmed-meshheading:8194193-Neoplasm Invasiveness, pubmed-meshheading:8194193-Oncogenes, pubmed-meshheading:8194193-Proteoglycans, pubmed-meshheading:8194193-Vimentin
pubmed:year
1994
pubmed:articleTitle
Oncogene-induced basement membrane invasiveness in human mammary epithelial cells.
pubmed:affiliation
Vincent T. Lombardi Cancer Research Center, Georgetown University Medical Center, Washington, DC 20007.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.