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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
1994-9-1
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pubmed:abstractText |
OBJECTIVES: Limulus anti-lipopolysaccharide factor, an 11.8-kilodalton peptide isolated from amebocytes of Limulus polyphemus inhibits the biologic activities of endotoxin in vitro, including gelation of Limulus amebocyte lysate. A recombinant version of Limulus anti-lipopolysaccharide factor, termed endotoxin neutralizing protein, has now been expressed in yeast. Endotoxin-neutralizing protein was evaluated for its potential prophylactic and therapeutic effects in rabbits challenged with Escherichia coli endotoxin. DESIGN: Controlled animal trial. SETTING: Animal research laboratory. SUBJECTS: A total of 112 New Zealand white rabbits were studied. INTERVENTIONS: Rabbits were challenged with an LD80 dose of E. coli endotoxin (100 micrograms/kg); control animals (n = 52) were treated with saline solution at the time of endotoxin challenge; experimental animals received endotoxin-neutralizing protein 2.5 mg/kg prechallenge (n = 20), 5.0 mg/kg prechallenge (n = 20), or 5.0 mg/kg 30 mins postchallenge (n = 20).
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anti-Bacterial Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Antimicrobial Cationic Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Arthropod Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Endotoxins,
http://linkedlifedata.com/resource/pubmed/chemical/Invertebrate Hormones,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/antilipopolysaccharide factor...,
http://linkedlifedata.com/resource/pubmed/chemical/endotoxin, Escherichia coli
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0090-3493
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
22
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1211-8
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:8045139-Animals,
pubmed-meshheading:8045139-Anti-Bacterial Agents,
pubmed-meshheading:8045139-Antimicrobial Cationic Peptides,
pubmed-meshheading:8045139-Arthropod Proteins,
pubmed-meshheading:8045139-Blood Gas Analysis,
pubmed-meshheading:8045139-Blood Pressure,
pubmed-meshheading:8045139-Disease Models, Animal,
pubmed-meshheading:8045139-Drug Evaluation, Preclinical,
pubmed-meshheading:8045139-Endotoxins,
pubmed-meshheading:8045139-Escherichia coli,
pubmed-meshheading:8045139-Escherichia coli Infections,
pubmed-meshheading:8045139-Invertebrate Hormones,
pubmed-meshheading:8045139-Lethal Dose 50,
pubmed-meshheading:8045139-Lipopolysaccharides,
pubmed-meshheading:8045139-Premedication,
pubmed-meshheading:8045139-Rabbits,
pubmed-meshheading:8045139-Recombinant Proteins,
pubmed-meshheading:8045139-Shock, Septic,
pubmed-meshheading:8045139-Survival Rate,
pubmed-meshheading:8045139-Time Factors,
pubmed-meshheading:8045139-Tumor Necrosis Factor-alpha
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pubmed:year |
1994
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pubmed:articleTitle |
Effect of a recombinant endotoxin-neutralizing protein on endotoxin shock in rabbits.
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pubmed:affiliation |
Department of Medicine, Children's Hospital, Harvard Medical School, Boston, MA 02115.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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