Linked Life Data

8043514

Source:http://linkedlifedata.com/resource/pubmed/id/8043514

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1994-8-31
pubmed:abstractText
A critical role for protein kinase C (PKC) in signal transduction events has been well established. On the other hand, emerging evidence also suggests a role for regulation of PKC levels in mediating long term cellular functions. In human leukemia cell line HL-60, the action of 1,25-dihydroxyvitamin D3 results in transcriptional up-regulation of PKC beta (within 8-12 h) (L. M. Obeid et al., J. Biol. Chem., 265: 2370-2374, 1990). In this study, the role of PKC beta in the regulation of proliferation and differentiation was studied. 1,25-Dihydroxyvitamin D3 caused an increase in PKC beta I, beta II, and, to a lesser extent, PKC alpha, as determined by Western blot analysis. This increase was accompanied by inhibition of proliferation and induction of differentiation. The addition of a 25-base pair antisense oligonucleotide directed against the 5' coding sequence of PKC beta attenuated up-regulation of PKC beta I and beta II levels in response to 1,25-dihydroxyvitamin D3. This antisense oligonucleotide, but not sense oligonucleotide or antisense oligonucleotide to PKC alpha, caused inhibition of 1,25-dihydroxyvitamin D3-induced differentiation by 25-45%. On the other hand, inhibition of cell proliferation by 1,25-dihydroxyvitamin D3 was minimally affected by the addition of antisense oligonucleotides. These results identify a role for PKC beta in cell differentiation and underscore the significance of transcriptional activation of PKC as a mechanism for long-term regulation of PKC. The results also distinguish signaling pathways involved in cell differentiation from those involved in antiproliferation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1044-9523
pubmed:author
pubmed:issnType
Print
pubmed:volume
5
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
405-9
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Specific role for protein kinase C beta in cell differentiation.
pubmed:affiliation
Division of Hematology/Oncology, Duke University Medical Center, Durham, North Carolina 27710.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.