Linked Life Data

7813397

Source:http://linkedlifedata.com/resource/pubmed/id/7813397

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1995-2-7
pubmed:abstractText
We present here a protocol for unidirectional wet electrotransfer of isoelectric focusing (IEF) gels to polyvinylidene difluoride (PVDF) that can be carried out in < 2 h, including preequilibration steps. In developing this protocol, we systematically evaluated sodium dodecyl sulfate and methanol concentrations in the preequilibration washes and length of electrotransfer. We demonstrated that the method is useful for tubulin, a protein that resists usual semi-dry transfer procedures due to its limited solubility under IEF conditions. We also successfully used our method for transferring the microtubule-associated protein, tau, as well as carbamylyte standards, creatine phosphokinase and carbonic anhydrase.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0173-0835
pubmed:author
pubmed:issnType
Print
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
930-1
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Method for rapid electrophoretic transfer of isoelectric focusing gels to polyvinylidene difluoride.
pubmed:affiliation
School of Nursing, Department of Biochemistry, University of Mississippi Medical Center, Jackson 39216.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.