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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1994-8-11
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pubmed:abstractText |
Cyclophosphamide induced fragmented nuclei in mouse thymic epithelial cells. Agarose gel electrophoresis showed the fragmentation of the DNA extracted from mouse thymus exposed to cyclophosphamide. The cell death induced by cyclophosphamide was considered to be apoptotic. Polyclonal antibody against single-stranded DNA was used immunohistochemically to detect apoptotic cell death in thymic epithelial cells. This antibody also detected programmed cell death in the interdigital necrotic zone of the mouse limb plate on day 14 of gestation, and in the ganglion of the trigeminal nerve on day 13 of gestation. These results show that the antibody specific for single-stranded DNA detected both drug-induced apoptosis and programmed cell death during embryogenesis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0301-5564
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
101
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
73-8
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pubmed:dateRevised |
2003-11-14
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pubmed:meshHeading |
pubmed-meshheading:7517929-Animals,
pubmed-meshheading:7517929-Antibodies,
pubmed-meshheading:7517929-Apoptosis,
pubmed-meshheading:7517929-Cell Death,
pubmed-meshheading:7517929-Cyclophosphamide,
pubmed-meshheading:7517929-DNA, Single-Stranded,
pubmed-meshheading:7517929-Dendritic Cells,
pubmed-meshheading:7517929-Eosine Yellowish-(YS),
pubmed-meshheading:7517929-Extremities,
pubmed-meshheading:7517929-Female,
pubmed-meshheading:7517929-Hematoxylin,
pubmed-meshheading:7517929-Immunohistochemistry,
pubmed-meshheading:7517929-Male,
pubmed-meshheading:7517929-Mice,
pubmed-meshheading:7517929-Mice, Inbred ICR,
pubmed-meshheading:7517929-Necrosis,
pubmed-meshheading:7517929-Pregnancy,
pubmed-meshheading:7517929-Staining and Labeling,
pubmed-meshheading:7517929-Trigeminal Ganglion
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pubmed:year |
1994
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pubmed:articleTitle |
Antibody against single-stranded DNA detects both programmed cell death and drug-induced apoptosis.
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pubmed:affiliation |
Department of Morphology, Institute for Developmental Research, Aichi Prefectural Colony, Japan.
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pubmed:publicationType |
Journal Article
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