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rdf:type |
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lifeskim:mentions |
umls-concept:C0004927,
umls-concept:C0007004,
umls-concept:C0008559,
umls-concept:C0009325,
umls-concept:C0023779,
umls-concept:C0025663,
umls-concept:C0026385,
umls-concept:C0032521,
umls-concept:C0205349,
umls-concept:C0456205,
umls-concept:C0520510,
umls-concept:C0680844,
umls-concept:C0681916,
umls-concept:C0750572,
umls-concept:C1550605,
umls-concept:C1707271,
umls-concept:C1998793
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pubmed:issue |
1
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pubmed:dateCreated |
1981-3-27
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pubmed:abstractText |
Polymeric cross-linked C-terminal peptide material (poly-alpha 1CB6) from mature bovine tendon type-I collagen was prepared and purified by a modification of the method previously described [Light & Bailey (1980) Biochem. J. 185, 373-381]. Poly-alpha 1CB6 was shown to exhibit concentration-dependent aggregation effects on gel filtration due to interaction with a filtration medium. The material had an amino acid content that was very similar to a mixture of alpha 1CB6 and alpha 1CB5. The material was shown to be polydisperse with a mol.wt. range of 50 000-350 000, but chromatographic fractions were relatively homogeneous over this molecular weight range with respect to amino-acid composition. The heterogeneity of the material was not due to incomplete CNBr peptide cleavage, as poly-alpha 1CB6 did not contain detectable quantities of methionine. The material showed no discrete bands on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis but gave a constant blue stain throughout the molecular weight range described above. Lipid analysis showed that the partially purified material contained elevated levels of stearate when compared to the crude CNBr-digested starting material. This may indicate the specific association of a stearic-acid-rich lipid with the peptide material. On carbohydrate analysis poly-alpha 1CB6 was shown to contain only galactose and glucose at levels of 0.72 and 0.28% respectively. The carbohydrate and amino acid analyses indicated that (alpha 1CB6)2-(alpha 1CB5)1 may be the basic cross-linked structural unit of poly-alpha 1CB6)2-(alpha 1CB5)1 units, although the carbohydrate analysis indicated that the higher molecular weight oligomers may be enriched in alpha 1CB6.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7458897-131672,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7458897-14193644,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7458897-4326447,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7458897-4354949,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7458897-456357,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7458897-458854,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7458897-4620384,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7458897-4722452,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7458897-5025627,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7458897-508799,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7458897-5102923,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7458897-5329026,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7458897-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7458897-5451907,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7458897-6249253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7458897-670217,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7458897-6769428,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7458897-761615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7458897-991856
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
189
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
111-24
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7458897-Amino Acids,
pubmed-meshheading:7458897-Animals,
pubmed-meshheading:7458897-Biopolymers,
pubmed-meshheading:7458897-Carbohydrates,
pubmed-meshheading:7458897-Cattle,
pubmed-meshheading:7458897-Chemical Phenomena,
pubmed-meshheading:7458897-Chemistry,
pubmed-meshheading:7458897-Chromatography, Gel,
pubmed-meshheading:7458897-Collagen,
pubmed-meshheading:7458897-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:7458897-Lipids,
pubmed-meshheading:7458897-Molecular Weight,
pubmed-meshheading:7458897-Peptides
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pubmed:year |
1980
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pubmed:articleTitle |
Polymeric C-terminal cross-linked material from type-I collagen. A modified method for purification, anomalous behaviour on gel filtration, molecular weight estimation, carbohydrate content and lipid content.
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pubmed:publicationType |
Journal Article
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