Linked Life Data

7441724

Source:http://linkedlifedata.com/resource/pubmed/id/7441724

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1981-2-24
pubmed:abstractText
The volume change of sarcoplasmic reticulum vesicles was induced by Ca2+ uptake. This volume change was measured by the light-scattering method. When vesicles were shrunk beforehand under the condition that anions are more permeable than cations, they swelled during Ca2+ uptake due to the concomitant incorporation of anions. On the contrary, they shrank with Ca2+ uptake due to the extrusion of cations under the condition that cations are more permeable than anions. From the analysis of the volume change it was concluded that all ions other than Ca2+ were transported passively in order to neutralize the membrane potential generated by the Ca2+ pump. These results support the idea that the Ca2+ pump is electrogenic. By using this technique, it became possible to measure the fast Ca2+ uptake rate. The dependence of the Ca2+ uptake rate on the Ca2+ concentration suggests that the site at which Ca2+ inhibits Ca2+ uptake is located inside the vesicle. From the osmotic response of the vesicles, the intravesicular concentration of free Ca2+ was estimated to be about 15 mM, when Ca2+ was fully taken up under the physiological condition.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0022-2631
pubmed:author
pubmed:issnType
Print
pubmed:day
30
pubmed:volume
56
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
159-68
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
Ion movement accompanied by calcium uptake of sarcoplasmic reticulum vesicles studied through the osmotic volume change by the light scattering method.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't