Linked Life Data

7419667

Source:http://linkedlifedata.com/resource/pubmed/id/7419667

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1980-12-16
pubmed:abstractText
A non-invasive method is described for estimating dopamine beta-hydroxylase (DBH) activity in sympathetic neurons. Our assay is based on the measurement of the in vivo rate of tritiated water (THO) release from dopamine specifically labeled in the beta-position ([beta-3H]DA). In validation of this method, we have establsihed in human subjects that 1) THO release from [beta-3H]DA correlates with [beta-3H]norepinephrine metabolite excretion, and 2) partial inhibition of DBH with disulfiram (5.5 mg/kg for 4 days) causes parallel decreases in these two indices of dopamine beta-hydroxylation. Since there is a strong correlation (r = 0.89; P < 0.05) between the effect of disulfiram on THO release and the effect of the drug on [3H]-norepinephrine metabolite excretion, our data indicate that the rate at which THO is liberated from [beta-3H]DA into the total body water can be used as an in vivo index of DBH activity.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0021-972X
pubmed:author
pubmed:issnType
Print
pubmed:volume
51
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
810-15
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
An in vivo tritium release assay of human dopamine beta-hydroxylase.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't