Linked Life Data

7190829

Source:http://linkedlifedata.com/resource/pubmed/id/7190829

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
14
pubmed:dateCreated
1980-11-24
pubmed:abstractText
The binding studies of the interaction of ethidium bromide with DNA have been hampered by its reversibility, which prevents direct isolation and thus characterization of the complex. The recent development of photoaffinity labeling has provided a means to circumvent this problem. However, to be useful as a probe for the parent compound, a photosensitive analogue must be shown to interact in vivo and in vitro just as the parent analogue. These studies demonstrate by steady-state and nonosecond fluorescence and stopped-flow kinetics that one of the azido analogues of ethidium, 8-azido-3-amino-5-ethyl-6-phenylphenanthridinium chloride, binds nucleic acids quite similarly to ethidium bromide. The interaction of the other azide, 3,8-diazido-5-ethyl-6-phenyl-phenanthridinium chloride, with DNA is qualitatively different from that of the monoazide and ethidium bromide. These results suggest that the monoazide would serve as an ideal probe for determining the actual target sites of ethidium bromide in vivo and in vitro.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
8
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3221-6
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1980
pubmed:articleTitle
Comparative studies of the binding of ethidium bromide and its photoreactive analogues to nucleic acids by fluorescence and rapid kinetics.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.