Linked Life Data

6616463

Source:http://linkedlifedata.com/resource/pubmed/id/6616463

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1983-11-23
pubmed:abstractText
The widely used drug cimetidine (Tagamet) can be nitrosated in the presence of nitrite and under mild acid conditions to form a compound, nitrosocimetidine (NC), which has a chemical structure very similar to those of the mutagens and laboratory carcinogens N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and methylnitrosourea (MNU). NC has given positive indications in several short-term tests for possible carcinogenic activity and is capable of methylating DNA in vitro and in cultured cells in a manner identical to that of MNNG and MNU. Nevertheless, NC has been found to be a weak carcinogen or a noncarcinogen and to be very poor at modifying DNA in vivo when administered p.o. We have found that NC, like MNNG, decomposes very rapidly when incubated with thiol compound in neutral pH buffer. Much of this decomposition is denitrosation. In the presence of excess reduced glutathione, about 35% of the degradation results in denitrosation to produce cimetidine, and in the presence of excess cysteine about 65% results in denitrosation to produce cimetidine. The compound also rapidly decomposes in whole blood isolated from rats; about 70% of this decomposition produces cimetidine. In solution with purified rat hemoglobin, approximately 90% of the NC degradation proceeds via a denitrosation pathway; hemoglobin cysteine residues have been implicated in the denitrosation reaction. In parallel experiments with MNNG, it has been found that, although a fraction of the decomposition of this agent in the presence of thiol compound, in isolated whole blood, and in solution with purified hemoglobin generates the denitrosated derivative, denitrosation is in the range of one-third to one-half of that found for NC. Denitrosation and degradation to form a methylating species appear to be the major NC and MNNG decomposition pathways in vitro. There is no indication that MNU degradation is sensitive to thiols, nor is the compound susceptible to denitrosation at neutral pH. Molar-equivalent doses of methyl group-radiolabeled NC, MNNG, and MNU were administered via the tail vein to groups of F344 rats, and the DNA methylation yields in lung, liver, kidney, and brain tissue were assessed. Of the organs considered, DNA methylation was greatest in the lungs of MNNG-treated animals, followed by kidney (25% of the lung value). Methylation of lung tissue DNA in MNU-treated animals was about 50% of that observed in the MNNG experiments; DNA methylation in the other organs was about equivalent to that found in the lung.(ABSTRACT TRUNCATED AT 400 WORDS)
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0008-5472
pubmed:author
pubmed:issnType
Print
pubmed:volume
43
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5258-67
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Denitrosation as a determinant of nitrosocimetidine in vivo activity.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.