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pubmed-article:6268047pubmed:abstractTextA whole-blood-culture technique was used to sequentially evaluated peripheral blood lymphocyte responses to phytohemagglutinin (PHA) and concanavalin A (Con A) of normal chickens and chickens infected at 1 day or 3 weeks of age with infectious bursal disease virus (IBDV). This method had numerous advantages over the more conventional techniques. A comparative study was made on the percentage of inhibition of responses of peripheral blood lymphocytes to PHA and Con A of 1-day- and 3-week-old IBDV-infected chickens. In both groups, there was a minimum inhibition between 3 and 4 weeks postinfection (PI) and a maximum inhibition at 6 weeks PI. A one-way mixed lymphocyte reaction (MLR) was performed using mitomycin-C-treated cells as stimulator cells obtained from chickens of genetically different strains. Lymphocytes from the experimental birds (control, 1-day-infected, and 3-week-infected groups) were used as the responder cells. The results showed that MLR response of the IBDV-infected chickens was significantly reduced compared with those of the uninfected controls. The degree of lowered response was much more severe in chickens infected at 1 day of age than in those infected at 3 weeks of age.lld:pubmed
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pubmed-article:6268047pubmed:authorpubmed-author:MaheswaranS...lld:pubmed
pubmed-article:6268047pubmed:authorpubmed-author:SivanandanVVlld:pubmed
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pubmed-article:6268047pubmed:pagination112-20lld:pubmed
pubmed-article:6268047pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:6268047pubmed:articleTitleImmune profile of infectious bursal disease. III. Effect of infectious bursal disease virus on the lymphocyte responses to phytomitogens and on mixed lymphocyte reaction of chickens.lld:pubmed
pubmed-article:6268047pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:6268047pubmed:publicationTypeComparative Studylld:pubmed
pubmed-article:6268047pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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