Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
25
pubmed:dateCreated
1972-4-6
pubmed:abstractText
A transition metal compound that is bound in tissues by any appropriate cytochemical reaction may catalyze the generation of an insoluble osmiophilic polymer from organic monomers such as 3,3'-diaminobenzidine. When the polymers are treated with osmium tetroxide, electron-opaque, insoluble osmium blacks (coordination polymers of osmium) are formed at the sites of the particular macromolecule or enzyme permitting its light, and electron, microscopic localization. This approach represents a distinct advantage over earlier cytochemical methods because the shorter incubation time needed here results in less artifactual deposition of metal ions, and less tendency to crystallize the reaction product. In addition, the shorter incubation times permit longer fixation of tissues and hence less artifact due to enzyme diffusion.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0036-8075
pubmed:author
pubmed:issnType
Print
pubmed:day
3
pubmed:volume
175
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
991-3
pubmed:dateRevised
2009-10-27
pubmed:meshHeading
pubmed-meshheading:5009401-Acid Phosphatase, pubmed-meshheading:5009401-Aniline Compounds, pubmed-meshheading:5009401-Animals, pubmed-meshheading:5009401-Biphenyl Compounds, pubmed-meshheading:5009401-Catalysis, pubmed-meshheading:5009401-Endoplasmic Reticulum, pubmed-meshheading:5009401-Esterases, pubmed-meshheading:5009401-Ferrocyanides, pubmed-meshheading:5009401-Histocytochemistry, pubmed-meshheading:5009401-Indicators and Reagents, pubmed-meshheading:5009401-Kidney Tubules, pubmed-meshheading:5009401-Lysosomes, pubmed-meshheading:5009401-Macromolecular Substances, pubmed-meshheading:5009401-Microscopy, Electron, pubmed-meshheading:5009401-Organometallic Compounds, pubmed-meshheading:5009401-Osmium, pubmed-meshheading:5009401-Phosphotungstic Acid, pubmed-meshheading:5009401-Polymers, pubmed-meshheading:5009401-Rats
pubmed:year
1972
pubmed:articleTitle
Osmiophilic polymer generation: catalysis by transition metal compounds in ultrastructural cytochemistry.
pubmed:publicationType
Journal Article