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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
1985-11-21
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pubmed:abstractText |
A 175 kdalton (kDa) polypeptide is bound covalently to the chromosomal DNA fragments from mouse cells exposed to the intercalating agent 4'-[(9-acridinyl)-amino]methansulphon-m-anisidide. Electron microscopy shows a terminal protein on the DNA fragments, whose 5'-termini are blocked. Since the relative molecular mass of topoisomerase II polypeptide chains is also about 175 kDa and topoisomerase II inhibitors prevent intercalator-induced DNA fragmentation, we propose that the polypeptide bound covalently to the 5'-terminus of the DNA fragments is a polypeptide derived from frequently integrated topoisomerase II operating to normalize torsional stress resulting from intercalation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0714-7511
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
63
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
780-3
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:3840052-Aminoacridines,
pubmed-meshheading:3840052-Amsacrine,
pubmed-meshheading:3840052-Animals,
pubmed-meshheading:3840052-Cell Line,
pubmed-meshheading:3840052-Chromosomes,
pubmed-meshheading:3840052-DNA,
pubmed-meshheading:3840052-Deoxyribonucleoproteins,
pubmed-meshheading:3840052-Intercalating Agents,
pubmed-meshheading:3840052-Mice,
pubmed-meshheading:3840052-Microscopy, Electron,
pubmed-meshheading:3840052-Molecular Weight
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pubmed:year |
1985
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pubmed:articleTitle |
Chromosomol DNA fragments from mouse cells exposed to an intercalating agent contain a 175-kdalton terminal polypeptide.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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