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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
1986-8-21
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pubmed:abstractText |
A negative selection procedure has been developed to obtain murine T helper clones specific for variable regions of the influenza A hemagglutinin. T cell lines, established from mice primed by intranasal infection with X31 (H3N2) virus, were cross-stimulated with natural variant viruses of known primary sequence (either A/TEXAS/1/77 or A/ENG878/69) and proliferating cells eliminated by treatment with the cell cycle-specific drug 5-bromodeoxyuridine. After two suicide cycles, T cell lines were subtype specific and failed to recognize the natural variants. Clones were established by limiting dilution and their specificity was determined against a panel of viruses. Extensive diversity was evident in the reactivity of clones from individual donors, and two major T cell recognition sites were defined in the globular head region of the hemagglutinin molecule.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0014-2980
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
789-93
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:2941307-Amino Acid Sequence,
pubmed-meshheading:2941307-Animals,
pubmed-meshheading:2941307-Antigen-Antibody Reactions,
pubmed-meshheading:2941307-Antigens, Viral,
pubmed-meshheading:2941307-Bromodeoxyuridine,
pubmed-meshheading:2941307-Cell Cycle,
pubmed-meshheading:2941307-Clone Cells,
pubmed-meshheading:2941307-H-2 Antigens,
pubmed-meshheading:2941307-Hemagglutinin Glycoproteins, Influenza Virus,
pubmed-meshheading:2941307-Hemagglutinins, Viral,
pubmed-meshheading:2941307-Influenza A virus,
pubmed-meshheading:2941307-Mice,
pubmed-meshheading:2941307-Mice, Inbred BALB C,
pubmed-meshheading:2941307-Mice, Inbred CBA,
pubmed-meshheading:2941307-Phenotype,
pubmed-meshheading:2941307-Rats,
pubmed-meshheading:2941307-Selection, Genetic,
pubmed-meshheading:2941307-T-Lymphocytes, Helper-Inducer
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pubmed:year |
1986
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pubmed:articleTitle |
Suicide selection of murine T helper clones specific for variable regions of the influenza hemagglutinin molecule.
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pubmed:publicationType |
Journal Article
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