| pubmed-article:2838272 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2838272 | lifeskim:mentions | umls-concept:C1882726 | lld:lifeskim |
| pubmed-article:2838272 | lifeskim:mentions | umls-concept:C0001455 | lld:lifeskim |
| pubmed-article:2838272 | lifeskim:mentions | umls-concept:C0031640 | lld:lifeskim |
| pubmed-article:2838272 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:2838272 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:2838272 | pubmed:dateCreated | 1988-8-4 | lld:pubmed |
| pubmed-article:2838272 | pubmed:abstractText | A low-Km cyclic nucleotide phosphodiesterase solubilised from rat liver membranes by mild proteolysis with chymotrypsin has been purified to apparent homogeneity. The purification included chromatography on cellulose phosphate, Ecteola-cellulose, hydroxyapatite, a theophylline affinity matrix and HPLC on a DEAE-substituted column. The purified enzyme has linear kinetic plots with a Km of 0.24 microM and a Vmax of 6.2 mumol mg-1 min-1 with cyclic AMP as a substrate. It also hydrolyses cyclic GMP with a Km of 0.17 microM and a Vmax which is about a third of that with cyclic AMP. Cyclic GMP is also a competitive inhibitor of cyclic AMP hydrolysis with a Ki of 0.18 microM. The proteolytically solubilised enzyme has a subunit molecular mass of 73 kDa by SDS gel electrophoresis and of 130 kDa by HPLC size-exclusion chromatography, suggesting that it exists as a dimer. A partially purified preparation of this enzyme was used to raise antiserum in a sheep. The antiserum immunoprecipitated activity from liver and adipose tissue of rat and mouse. It had little activity against phosphodiesterase from other rat tissues or other species. Insulin-activated phosphodiesterase from both adipocytes and hepatocytes was immunoprecipitated by the antiserum suggesting that the purified enzyme was an insulin-sensitive phosphodiesterase. | lld:pubmed |
| pubmed-article:2838272 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2838272 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2838272 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2838272 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2838272 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2838272 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2838272 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2838272 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:2838272 | pubmed:issn | 0014-2956 | lld:pubmed |
| pubmed-article:2838272 | pubmed:author | pubmed-author:LotenE GEG | lld:pubmed |
| pubmed-article:2838272 | pubmed:author | pubmed-author:BoyerPP | lld:pubmed |
| pubmed-article:2838272 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2838272 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:2838272 | pubmed:volume | 174 | lld:pubmed |
| pubmed-article:2838272 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2838272 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2838272 | pubmed:pagination | 303-9 | lld:pubmed |
| pubmed-article:2838272 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
| pubmed-article:2838272 | pubmed:meshHeading | pubmed-meshheading:2838272-... | lld:pubmed |
| pubmed-article:2838272 | pubmed:meshHeading | pubmed-meshheading:2838272-... | lld:pubmed |
| pubmed-article:2838272 | pubmed:meshHeading | pubmed-meshheading:2838272-... | lld:pubmed |
| pubmed-article:2838272 | pubmed:meshHeading | pubmed-meshheading:2838272-... | lld:pubmed |
| pubmed-article:2838272 | pubmed:meshHeading | pubmed-meshheading:2838272-... | lld:pubmed |
| pubmed-article:2838272 | pubmed:meshHeading | pubmed-meshheading:2838272-... | lld:pubmed |
| pubmed-article:2838272 | pubmed:meshHeading | pubmed-meshheading:2838272-... | lld:pubmed |
| pubmed-article:2838272 | pubmed:meshHeading | pubmed-meshheading:2838272-... | lld:pubmed |
| pubmed-article:2838272 | pubmed:meshHeading | pubmed-meshheading:2838272-... | lld:pubmed |
| pubmed-article:2838272 | pubmed:meshHeading | pubmed-meshheading:2838272-... | lld:pubmed |
| pubmed-article:2838272 | pubmed:year | 1988 | lld:pubmed |
| pubmed-article:2838272 | pubmed:articleTitle | Purification of an insulin-sensitive cyclic AMP phosphodiesterase from rat liver. | lld:pubmed |
| pubmed-article:2838272 | pubmed:affiliation | Department of Clinical Biochemistry, University of Otago Medical School, Dunedin, New Zealand. | lld:pubmed |
| pubmed-article:2838272 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2838272 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2838272 | lld:pubmed |
