Linked Life Data

2472815

Source:http://linkedlifedata.com/resource/pubmed/id/2472815

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1989-8-22
pubmed:abstractText
A porcine brain microvessel-derived cDNA library enriched in blood-brain-barrier-specific sequences was constructed by a subtractive cloning procedure. Two cDNA clones from this library were found to encode a glucose transport protein. These clones were used to isolate a nearly full length cDNA from a representative brain microvessel library. Analysis of the amino-acid sequence deduced from the cDNA sequence revealed 97% identity with the human HepG2 glucose carrier. Amino-acid substitutions appear to be clustered in certain regions of the polypeptide. The nucleotide sequences of the 3'-noncoding regions close to the putative polyadenylation sites are highly conserved in glucose transporter mRNAs of different species. The expression of this mRNA has been investigated in various tissues and shown to be decreased in primary cultures of brain microvascular endothelial cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0177-3593
pubmed:author
pubmed:issnType
Print
pubmed:volume
370
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
467-73
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
cDNA cloning and sequence analysis of the glucose transporter from porcine blood-brain barrier.
pubmed:affiliation
Institut für Biochemie, Technische Hochschule Darmstadt.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't