Linked Life Data

2407122

Source:http://linkedlifedata.com/resource/pubmed/id/2407122

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1990-3-26
pubmed:abstractText
In our previous report, we described a new fixation and paraffin-embedding method (the AMeX method) that preserves many of the antigens that are normally destroyed by routine formalin fixation. The current study was conducted to examine the preservation of high-molecular-weight DNA in tissues processed by this method. DNA was extracted from AMeX-processed tissue sections after deparaffinization by the same method as that used to extract DNA from fresh tissues. The total amounts of DNA extracted from 10 mg each in wet weight of AMeX-processed and fresh mouse liver tissues were identical. In tissues of malignant lymphoma, the total amount of spooled DNA extracted from 50 sections, each 20 microns thick, was about 8 micrograms/mm2. The electrophoretic pattern of DNA digested with restriction endonucleases on agarose gel from AMeX-processed tissue sections did not differ from that of fresh materials. Southern blot hybridization analysis also revealed that the mobility of specific DNA fragments was identical for AMeX-processed and fresh tissues. The AMeX method was thus proved to be a versatile multipurpose tissue-processing procedure, which is expected to provide important information regarding the correlation between morphology, phenotypic expression, and gene alteration.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2407122-1195397, http://linkedlifedata.com/resource/pubmed/commentcorrection/2407122-2432790, http://linkedlifedata.com/resource/pubmed/commentcorrection/2407122-2992457, http://linkedlifedata.com/resource/pubmed/commentcorrection/2407122-3009004, http://linkedlifedata.com/resource/pubmed/commentcorrection/2407122-3014659, http://linkedlifedata.com/resource/pubmed/commentcorrection/2407122-3411933, http://linkedlifedata.com/resource/pubmed/commentcorrection/2407122-3941898, http://linkedlifedata.com/resource/pubmed/commentcorrection/2407122-4047115, http://linkedlifedata.com/resource/pubmed/commentcorrection/2407122-6402307, http://linkedlifedata.com/resource/pubmed/commentcorrection/2407122-6695174, http://linkedlifedata.com/resource/pubmed/commentcorrection/2407122-6719137
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0002-9440
pubmed:author
pubmed:issnType
Print
pubmed:volume
136
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
267-71
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
The AMeX method: a multipurpose tissue-processing and paraffin-embedding method. II. Extraction of spooled DNA and its application to Southern blot hybridization analysis.
pubmed:affiliation
Pathology Division, National Cancer Center Research Institute, Tokyo, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't