|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
6
|
|
pubmed:dateCreated |
1990-7-13
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
The major nuclear pore protein p62 is modified by O-linked N-acetylglucosamine and functions in nuclear transport. We have cloned, sequenced, and expressed the full-length rat p62 cDNA. The rat p62 mRNA is 2,941 nucleotides long and encodes a protein of 525 amino acids containing 30% serine and threonine residues. The amino acid sequence near the amino-terminus contains unique tetrapeptide repeats while the carboxy-terminus consists of a series of predicted alpha-helical regions with hydrophobic heptad repeats. Heterologous expression of rat p62 in African Green Monkey Kidney COS-1 cells and CV-1 cells was detected using a species-specific antipeptide serum. When transiently expressed in COS-1 cells, rat p62 binds wheat germ agglutinin and concentrates at the spindle poles during mitosis. In CV-1 cells cotransfected with rat p62 cDNA and SV40 viral DNA, rat p62 associates with the nuclear membrane without interfering with the nuclear transport of SV40 large T antigen. The ability to express p62 in tissue culture cells will facilitate analysis of the role of this pore protein in nuclear transport.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2190987-2295087,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2190987-2437126,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2190987-2442757,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2190987-2459127,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2190987-3064988,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2190987-3110163,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2190987-3139301,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2190987-3169133,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2190987-3200844,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2190987-3294718,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2190987-3309563,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2190987-3313397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2190987-3345567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2190987-3353225,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2190987-3518946,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2190987-3549279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2190987-3571327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2190987-3657580,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2190987-3805121,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2190987-3838905,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2190987-3943125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2190987-5924199,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2190987-6246378,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2190987-6389413,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2190987-642007,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2190987-6892800
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jun
|
|
pubmed:issn |
0021-9525
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
110
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1861-71
|
|
pubmed:dateRevised |
2010-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:2190987-Amino Acid Sequence,
pubmed-meshheading:2190987-Animals,
pubmed-meshheading:2190987-Antigens, Polyomavirus Transforming,
pubmed-meshheading:2190987-Base Sequence,
pubmed-meshheading:2190987-Cell Line,
pubmed-meshheading:2190987-Cercopithecus aethiops,
pubmed-meshheading:2190987-Chromosome Mapping,
pubmed-meshheading:2190987-Cloning, Molecular,
pubmed-meshheading:2190987-DNA,
pubmed-meshheading:2190987-Fibroblasts,
pubmed-meshheading:2190987-Fluorescent Antibody Technique,
pubmed-meshheading:2190987-Immune Sera,
pubmed-meshheading:2190987-Kidney,
pubmed-meshheading:2190987-Membrane Glycoproteins,
pubmed-meshheading:2190987-Membrane Proteins,
pubmed-meshheading:2190987-Mitotic Spindle Apparatus,
pubmed-meshheading:2190987-Molecular Sequence Data,
pubmed-meshheading:2190987-Nuclear Envelope,
pubmed-meshheading:2190987-Nuclear Pore Complex Proteins,
pubmed-meshheading:2190987-Rats,
pubmed-meshheading:2190987-Transfection
|
|
pubmed:year |
1990
|
|
pubmed:articleTitle |
Primary sequence and heterologous expression of nuclear pore glycoprotein p62.
|
|
pubmed:affiliation |
Laboratory of Biochemistry and Metabolism, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
