|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
8
|
|
pubmed:dateCreated |
1990-8-21
|
|
pubmed:abstractText |
The receptor for mouse hepatitis virus strain A59 (MHV-A59) is a 110- to 120-kilodalton (kDa) glycoprotein which is expressed in MHV-susceptible mouse strains on the membranes of hepatocytes, intestinal epithelial cells, and macrophages. SJL/J mice, which are highly resistant to MHV-A59, were previously shown to lack detectable levels of receptor by using either solid-phase virus receptor assays or binding of a monoclonal anti-receptor antibody (MAb) which blocks infection of MHV-susceptible mouse cells. This MAb was used for affinity purification of the receptor glycoprotein from livers of MHV-susceptible Swiss Webster mice. The MHV receptor and an antigenically related protein of 48 to 58 kDa were copurified and then separated by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The first 15 amino acids of the receptor were sequenced, and a synthetic peptide of this amino acid sequence was prepared. Rabbit antiserum made against this peptide bound to the MHV receptor glycoprotein and the 48- to 58-kDa protein from livers of MHV-susceptible BALB/c mice and Swiss Webster mice and from the intestinal brush border of BALB/c mice. In immunoblots of intestinal brush border and hepatocyte membranes of MHV-resistant SJL/J mice, the antibody against the amino terminus of the receptor identified proteins that are 5 to 10 kDa smaller than the MHV receptor and the 48- to 58-kDa related protein from Swiss Webster or BALB/c mice. Thus, SJL/J mice express a protein which shares some sequence homology with the MHV receptor but which lacks virus-binding activity and is not recognized by the blocking anti-receptor MAb. These results suggest that resistance of SJL/J mice to MHV-A59 may be due to absence or mutation of the virus-binding domain in the nonfunctional receptor homolog in SJL/J mice.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2164599-135262,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2164599-14450081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2164599-163277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2164599-16590714,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2164599-236308,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2164599-2409098,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2164599-2547994,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2164599-2579095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2164599-2845655,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2164599-2985806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2164599-3020124,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2164599-3023696,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2164599-3379044,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2164599-3380803,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2164599-3611052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2164599-3718235,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2164599-3838350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2164599-388439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2164599-4097142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2164599-6088681,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2164599-6146650,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2164599-6178564,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2164599-620021,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2164599-6205117,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2164599-6245243,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2164599-6265583,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2164599-6277284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2164599-6286761,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2164599-6327825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2164599-6331343,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2164599-6855576,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2164599-942051
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Aug
|
|
pubmed:issn |
0022-538X
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
64
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
3817-23
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:2164599-Amino Acid Sequence,
pubmed-meshheading:2164599-Animals,
pubmed-meshheading:2164599-Antibodies, Monoclonal,
pubmed-meshheading:2164599-Cell Transformation, Viral,
pubmed-meshheading:2164599-Cells, Cultured,
pubmed-meshheading:2164599-Disease Susceptibility,
pubmed-meshheading:2164599-Female,
pubmed-meshheading:2164599-Hepatitis, Viral, Animal,
pubmed-meshheading:2164599-Intestines,
pubmed-meshheading:2164599-Liver,
pubmed-meshheading:2164599-Membrane Glycoproteins,
pubmed-meshheading:2164599-Mice,
pubmed-meshheading:2164599-Mice, Inbred BALB C,
pubmed-meshheading:2164599-Mice, Inbred Strains,
pubmed-meshheading:2164599-Microvilli,
pubmed-meshheading:2164599-Molecular Weight,
pubmed-meshheading:2164599-Murine hepatitis virus,
pubmed-meshheading:2164599-Receptors, Virus
|
|
pubmed:year |
1990
|
|
pubmed:articleTitle |
Purification of the 110-kilodalton glycoprotein receptor for mouse hepatitis virus (MHV)-A59 from mouse liver and identification of a nonfunctional, homologous protein in MHV-resistant SJL/J mice.
|
|
pubmed:affiliation |
Department of Pathology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|
