Linked Life Data

20875052

Source:http://linkedlifedata.com/resource/pubmed/id/20875052

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2010-11-9
pubmed:abstractText
Macrophage apoptosis represents an important innate defense mechanism against intracellular mycobacterial infection. Previous publications have shown that interferon-? (IFN-?) is involved in apoptosis of immune cells infected with mycobacteria. In this study, the impact of IFN-? treatment on phorbol-12-myristate-13-acetate-differentiated THP-1 cells infected with Mycobacterium bovis was investigated. The results showed that IFN-? increased apoptosis of THP-1 cells infected with M. bovis at a low multiplicity of infection (MOI) in a time-dependent manner. The percentage of cells undergoing apoptosis in IFN-?-treated THP-1 cells increased from 4.3% at 12 h to 36.5% at 72 h upon infection with an MOI of 10. Activation of caspases-3 and -8 increased 8.3- and 6.7-fold, respectively. Neutralizing endogenous tumor necrosis factor-? (TNF-?) significantly inhibited IFN-?-induced apoptosis of M. bovis-infected THP-1 cells. No significant change in IFN-?-induced apoptosis was observed in M. bovis-infected cells after the addition of c-Jun N-terminal kinase and NF-?B pathways' inhibitors. Translocation of apoptosis-inducing factor (AIF) to the nucleus of M. bovis-infected THP-1 cells was observed in 23.4% of IFN-?-treated cells, compared with 11.0% in untreated cells. Taken together, these results suggest that IFN-? promotes apoptosis of M. bovis-infected THP-1 cells during early infection through the TNF-?-mediated death receptor and the AIF apoptotic pathway.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1574-695X
pubmed:author
pubmed:copyrightInfo
© 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
pubmed:issnType
Electronic
pubmed:volume
60
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
191-8
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
IFN-? promotes THP-1 cell apoptosis during early infection with Mycobacterium bovis by activating different apoptotic signaling.
pubmed:affiliation
State Key Lab of Agrobiotechnology, College of Veterinary Medicine, China Agricultural University, Beijing, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't