Linked Life Data

20798218

Source:http://linkedlifedata.com/resource/pubmed/id/20798218

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
20
pubmed:dateCreated
2010-10-15
pubmed:abstractText
Inflammatory bowel diseases (IBD) increase the risk of developing colorectal cancer. Dietary components that reduce inflammation are associated with lower cancer risk. The long-chain omega-3 fatty acid docosahexaenoic acid (DHA) is present in fish oil and has potent anti-inflammatory properties. The objective of this study is to determine whether dietary fish oil enriched with DHA (DFO) could reduce experimentally induced colitis and colon cancer risk in a mouse model. When SMAD3-/- mice are exposed to Helicobacter hepaticus, mild colitis is observed 4 weeks postinfection. Mice were fed isocaloric diets modified to include corn oil, safflower oil, or DFO (doses ranging from 0.75% to 6.00%) as the fatty acid source for 8 weeks. Mice were gavaged with H. hepaticus; DFO feeding was continued; and mice were sacrificed 4 weeks after infection. The colon and cecum were collected for histopathology. Spleens and mesenteric lymph nodes were collected and analyzed for T-cell populations using flow cytometry. Contrary to expectations, DFO induced severe colitis and adenocarcinoma formation. DFO consumption was associated with decreased CD8(+) cell frequency and diminished CD69 expression on CD4(+) and CD8(+) T-cell populations. Mice consuming DFO also exhibited higher FoxP3(+) CD25(+) CD4(+) T regulatory cell frequency, FoxP3 expression, and altered L-selectin expression during infection. We concluded that DFO-fed mice may be less equipped to mount a successful response to H. hepaticus infection, increasing colon cancer risk. These results support the need to establish a tolerable upper limit for DHA intake particularly in the context of chronic inflammatory conditions such as IBD.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1538-7445
pubmed:author
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
70
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7960-9
pubmed:dateRevised
2011-1-19
pubmed:meshHeading
pubmed-meshheading:20798218-Animals, pubmed-meshheading:20798218-Antigens, CD, pubmed-meshheading:20798218-Antigens, Differentiation, T-Lymphocyte, pubmed-meshheading:20798218-CD4-Positive T-Lymphocytes, pubmed-meshheading:20798218-CD8-Positive T-Lymphocytes, pubmed-meshheading:20798218-Colitis, pubmed-meshheading:20798218-Colon, pubmed-meshheading:20798218-Colonic Neoplasms, pubmed-meshheading:20798218-Corn Oil, pubmed-meshheading:20798218-Dietary Fats, Unsaturated, pubmed-meshheading:20798218-Disease Models, Animal, pubmed-meshheading:20798218-Docosahexaenoic Acids, pubmed-meshheading:20798218-Fish Oils, pubmed-meshheading:20798218-Flow Cytometry, pubmed-meshheading:20798218-Inflammation, pubmed-meshheading:20798218-Lectins, C-Type, pubmed-meshheading:20798218-Mice, pubmed-meshheading:20798218-Mice, Knockout, pubmed-meshheading:20798218-Rectum, pubmed-meshheading:20798218-Smad3 Protein, pubmed-meshheading:20798218-T-Lymphocytes
pubmed:year
2010
pubmed:articleTitle
Dietary fish oil alters T lymphocyte cell populations and exacerbates disease in a mouse model of inflammatory colitis.
pubmed:affiliation
Department of Food Science and Human Nutrition, Department of Pathobiology and Diagnostic Investigation, and College of Osteopathic Medicine, Michigan State University, East Lansing, Michigan, USA.
pubmed:publicationType
Journal Article