| pubmed-article:2066384 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2066384 | lifeskim:mentions | umls-concept:C0019169 | lld:lifeskim |
| pubmed-article:2066384 | lifeskim:mentions | umls-concept:C0521009 | lld:lifeskim |
| pubmed-article:2066384 | lifeskim:mentions | umls-concept:C0439158 | lld:lifeskim |
| pubmed-article:2066384 | lifeskim:mentions | umls-concept:C0028953 | lld:lifeskim |
| pubmed-article:2066384 | lifeskim:mentions | umls-concept:C0220908 | lld:lifeskim |
| pubmed-article:2066384 | lifeskim:mentions | umls-concept:C0162326 | lld:lifeskim |
| pubmed-article:2066384 | lifeskim:mentions | umls-concept:C0182400 | lld:lifeskim |
| pubmed-article:2066384 | lifeskim:mentions | umls-concept:C0439831 | lld:lifeskim |
| pubmed-article:2066384 | lifeskim:mentions | umls-concept:C0075804 | lld:lifeskim |
| pubmed-article:2066384 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:2066384 | pubmed:dateCreated | 1991-8-15 | lld:pubmed |
| pubmed-article:2066384 | pubmed:abstractText | Transfection of the hepatitis B virus (HBV) genome requires the cloning of tandem HBV sequences into a plasmid vector, which is usually screened for by restriction enzyme digestion of plasmid minipreparations from at least a dozen bacterial colonies. We describe a simple alternative screening method based on in situ hybridization of bacterial colonies with a [32P]-labelled synthetic oligonucleotide which spans the head-to-tail junction site of two tandem HBV molecules. The accurate detection by the oligoprobe is confirmed by enzymatic digestion. | lld:pubmed |
| pubmed-article:2066384 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2066384 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2066384 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2066384 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2066384 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2066384 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:2066384 | pubmed:issn | 0166-0934 | lld:pubmed |
| pubmed-article:2066384 | pubmed:author | pubmed-author:VitvitskiLL | lld:pubmed |
| pubmed-article:2066384 | pubmed:author | pubmed-author:TrépoCC | lld:pubmed |
| pubmed-article:2066384 | pubmed:author | pubmed-author:EilHH | lld:pubmed |
| pubmed-article:2066384 | pubmed:author | pubmed-author:BenjellounSS | lld:pubmed |
| pubmed-article:2066384 | pubmed:author | pubmed-author:TongS PSP | lld:pubmed |
| pubmed-article:2066384 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2066384 | pubmed:volume | 32 | lld:pubmed |
| pubmed-article:2066384 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2066384 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2066384 | pubmed:pagination | 109-14 | lld:pubmed |
| pubmed-article:2066384 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:2066384 | pubmed:meshHeading | pubmed-meshheading:2066384-... | lld:pubmed |
| pubmed-article:2066384 | pubmed:meshHeading | pubmed-meshheading:2066384-... | lld:pubmed |
| pubmed-article:2066384 | pubmed:meshHeading | pubmed-meshheading:2066384-... | lld:pubmed |
| pubmed-article:2066384 | pubmed:meshHeading | pubmed-meshheading:2066384-... | lld:pubmed |
| pubmed-article:2066384 | pubmed:meshHeading | pubmed-meshheading:2066384-... | lld:pubmed |
| pubmed-article:2066384 | pubmed:meshHeading | pubmed-meshheading:2066384-... | lld:pubmed |
| pubmed-article:2066384 | pubmed:meshHeading | pubmed-meshheading:2066384-... | lld:pubmed |
| pubmed-article:2066384 | pubmed:meshHeading | pubmed-meshheading:2066384-... | lld:pubmed |
| pubmed-article:2066384 | pubmed:meshHeading | pubmed-meshheading:2066384-... | lld:pubmed |
| pubmed-article:2066384 | pubmed:meshHeading | pubmed-meshheading:2066384-... | lld:pubmed |
| pubmed-article:2066384 | pubmed:meshHeading | pubmed-meshheading:2066384-... | lld:pubmed |
| pubmed-article:2066384 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:2066384 | pubmed:articleTitle | Rapid screening for bacterial colonies harbouring tandem hepatitis B virus sequences by an oligonucleotide probe. | lld:pubmed |
| pubmed-article:2066384 | pubmed:affiliation | Unité de Recherche Sur Les Hépatites INSERM 271, Lyon, France. | lld:pubmed |
| pubmed-article:2066384 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2066384 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2066384 | lld:pubmed |
