Linked Life Data

20583058

Source:http://linkedlifedata.com/resource/pubmed/id/20583058

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
27
pubmed:dateCreated
2010-7-19
pubmed:abstractText
A novel electrochemical platform was designed by combining the biocompatibility of single-stranded DNA (ss-DNA) and the excellent conductivity of graphene (GP). This nanocomposite (denoted as ss-DNA/GP) was first used as an electrode material for the immobilization and biosensing of redox enzymes. On the basis of electrostatic interactions, horseradish peroxidase (HRP) self-assembled with ss-DNA/GP on the surface of a glassy carbon (GC) electrode to form an HRP/ss-DNA/GP/GC electrode. UV/Vis and FTIR spectra were used to monitor the assembly process and indicated that the immobilized HRP on the ss-DNA/GP matrix retained its native structure well. A pair of stable and well-defined redox peaks of HRP with a formal potential of about -0.26 V (vs. Ag/AgCl) in a pH 7.0 phosphate buffer solution were obtained at the HRP/ss-DNA/GP/GC electrode; this demonstrates direct electron transfer between the immobilized HRP and the electrode. In addition, the modified electrode showed good electrocatalytic performance towards H(2)O(2) with high sensitivity, wide linear range, and good stability. Accordingly, the ss-DNA/GP nanocomposite provides a novel and efficient platform for the immobilized redox enzyme to realize direct electrochemistry and has a promising application in the fabrication of third-generation electrochemical biosensors.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1521-3765
pubmed:author
pubmed:issnType
Electronic
pubmed:day
19
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8133-9
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Fabrication of a biocompatible and conductive platform based on a single-stranded DNA/graphene nanocomposite for direct electrochemistry and electrocatalysis.
pubmed:affiliation
Liaoning Provincial Key Laboratory for Green Synthesis and Preparative Chemistry of Advanced Materials, Liaoning University, Shenyang 110036, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't