Linked Life Data

20113467

Source:http://linkedlifedata.com/resource/pubmed/id/20113467

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2010-3-3
pubmed:abstractText
Sothern blotting is a DNA analysis technique that has found widespread application in molecular biology. It has been used for gene discovery and mapping and has diagnostic and forensic applications, including mutation detection in patient samples and DNA fingerprinting in criminal investigations. Southern blotting has been employed as the definitive method for detecting transgene integration, and successful homologous recombination in gene targeting experiments.The technique employs a labeled DNA probe to detect a specific DNA sequence in a complex DNA sample that has been separated by restriction-digest and gel electrophoresis. Critically for the technique to succeed the probe must be unique to the target locus so as not to cross-hybridize to other endogenous DNA within the sample.Investigators routinely employ a manual approach to probe design. A genome browser is used to extract DNA sequence from the locus of interest, which is searched against the target genome using a BLAST-like tool. Ideally a single perfect match is obtained to the target, with little cross-reactivity caused by homologous DNA sequence present in the genome and/or repetitive and low-complexity elements in the candidate probe. This is a labor intensive process often requiring several attempts to find a suitable probe for laboratory testing.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/20113467-11724738, http://linkedlifedata.com/resource/pubmed/commentcorrection/20113467-1195397, http://linkedlifedata.com/resource/pubmed/commentcorrection/20113467-12368254, http://linkedlifedata.com/resource/pubmed/commentcorrection/20113467-12427827, http://linkedlifedata.com/resource/pubmed/commentcorrection/20113467-14630221, http://linkedlifedata.com/resource/pubmed/commentcorrection/20113467-15123588, http://linkedlifedata.com/resource/pubmed/commentcorrection/20113467-15713233, http://linkedlifedata.com/resource/pubmed/commentcorrection/20113467-16796549, http://linkedlifedata.com/resource/pubmed/commentcorrection/20113467-19033362, http://linkedlifedata.com/resource/pubmed/commentcorrection/20113467-2162051, http://linkedlifedata.com/resource/pubmed/commentcorrection/20113467-2231712
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1471-2164
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
74
pubmed:dateRevised
2010-9-28
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Automated design of genomic Southern blot probes.
pubmed:affiliation
Genes to Cognition Programme, The Wellcome Trust Sanger Institute, Hinxton, Cambridge CB101SA, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Validation Studies