Source:http://linkedlifedata.com/resource/pubmed/id/19633924
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
2010-3-11
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pubmed:abstractText |
In the present study changes of components of Wnt signaling pathway--axin (AXIN1) and beta-catenin (CTNNB1) in a sample of 72 neuroepithelial brain tumors were investigated. AXIN-1 gene was tested by PCR/loss of heterozygosity (LOH). Immunostaining and image analysis revealed the quantity and localization of relevant proteins. Polymorphic marker for AXIN-1, showed LOH in 11.1% of tumors. LOH was distributed to 6.3% of glioblastomas, one was found in neuroepithelial dysembrioplastic tumor and one in medulloblastoma. Down regulation of axin expression and up regulation of beta-catenin were detected in the analyzed tumors. Axin was observed in the cytoplasm in 68.8% of samples, in 28.1% in both the cytoplasm and nucleus and 3.1% had no expression. Beta-catenin was observed mainly in the nucleus and cytoplasm (59.4%). Expression in 34.4% of samples was in the cytoplasm and 6.3% showed no expression. Comparison of mean values of relative increase of axin and beta-catenin showed that they are significantly reversely proportional (P = 0.014). Relative quantity of beta-catenin in patients with gross deletion of AXIN1 was significantly higher in comparison to patients without LOH (P = 0.040). Our results demonstrate that changes of key components of the Wnt signaling play a role in neuroepithelial brain tumors.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/AXIN1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Axin Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Wnt Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/beta Catenin
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1532-2807
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
75-9
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:19633924-Axin Protein,
pubmed-meshheading:19633924-Brain Neoplasms,
pubmed-meshheading:19633924-Gene Expression,
pubmed-meshheading:19633924-Gene Expression Profiling,
pubmed-meshheading:19633924-Humans,
pubmed-meshheading:19633924-Image Interpretation, Computer-Assisted,
pubmed-meshheading:19633924-Immunohistochemistry,
pubmed-meshheading:19633924-Loss of Heterozygosity,
pubmed-meshheading:19633924-Neoplasms, Neuroepithelial,
pubmed-meshheading:19633924-Polymerase Chain Reaction,
pubmed-meshheading:19633924-Repressor Proteins,
pubmed-meshheading:19633924-Signal Transduction,
pubmed-meshheading:19633924-Wnt Proteins,
pubmed-meshheading:19633924-beta Catenin
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pubmed:year |
2010
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pubmed:articleTitle |
Changes of AXIN-1 and beta-catenin in neuroepithelial brain tumors.
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pubmed:affiliation |
Laboratory of Neurooncology, Croatian Institute for Brain Research, School of Medicine University of Zagreb, Salata 12, 10000, Zagreb, Croatia.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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