Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
21
pubmed:dateCreated
2009-5-27
pubmed:abstractText
The US3 protein is a viral serine/threonine kinase that is conserved among all members of the Alphaherpesvirinae. The US3 protein of different alphaherpesviruses causes dramatic alterations in the actin cytoskeleton, such as the disassembly of actin stress fibers and formation of cell projections, which have been associated with increased intercellular virus spread. Here, we find that inhibiting group A p21-activated kinases (PAKs), which are key regulators in Cdc42/Rac1 Rho GTPase signaling pathways, impairs US3-mediated actin alterations. By using PAK1(-/-) and PAK2(-/-) mouse embryo fibroblasts (MEFs), we show that US3-mediated stress fiber disassembly requires PAK2, whereas US3-mediated cell projection formation mainly is mediated by PAK1, also indicating that PAK1 and PAK2 can have different biological effects on the organization of the actin cytoskeleton. In addition, US3 was found to bind and phosphorylate group A PAKs. Lack of group A PAKs in MEFs was correlated with inefficient virus spread. Thus, US3 induces its effect on the actin cytoskeleton via group A PAKs.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-10330410, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-10394361, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-10607567, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-10995762, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-11168588, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-11602726, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-12478284, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-12560339, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-12676796, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-12885923, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-15215691, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-15254172, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-15331659, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-15582661, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-15749698, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-15767401, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-15951429, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-16148307, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-16288885, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-16352537, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-16424340, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-16492808, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-16497585, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-16820319, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-17079291, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-17312971, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-17499381, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-17708935, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-17764949, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-18005700, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-18005701, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-18184710, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-18193035, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-18199048, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-18386044, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-18411304, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-18420139, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-18431401, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-18639460, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-18923409, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-19124833, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-19136132, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-7592896, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-8107774, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-8994833, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-9032240, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-9171063, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-9395435, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-9438836, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-9451000, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-9501066, http://linkedlifedata.com/resource/pubmed/commentcorrection/19435845-9528787
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1091-6490
pubmed:author
pubmed:issnType
Electronic
pubmed:day
26
pubmed:volume
106
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8707-12
pubmed:dateRevised
2010-9-27
pubmed:meshHeading
pubmed:year
2009
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