| pubmed-article:19118632 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19118632 | lifeskim:mentions | umls-concept:C0318256 | lld:lifeskim |
| pubmed-article:19118632 | lifeskim:mentions | umls-concept:C0680730 | lld:lifeskim |
| pubmed-article:19118632 | lifeskim:mentions | umls-concept:C0678594 | lld:lifeskim |
| pubmed-article:19118632 | lifeskim:mentions | umls-concept:C1148554 | lld:lifeskim |
| pubmed-article:19118632 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:19118632 | pubmed:dateCreated | 2009-3-2 | lld:pubmed |
| pubmed-article:19118632 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19118632 | pubmed:abstractText | Pseudopilins form the central pseudopilus of the sophisticated bacterial type 2 secretion systems. The crystallization of the EpsI:EpsJ pseudopilin heterodimer from Vibrio vulnificus was greatly accelerated by the use of nanobodies, which are the smallest antigen-binding fragments derived from heavy-chain only camelid antibodies. Seven anti-EpsI:EpsJ nanobodies were generated and co-crystallization of EpsI:EpsJ nanobody complexes yielded several crystal forms very rapidly. In the structure solved, the nanobodies are arranged in planes throughout the crystal lattice, linking layers of EpsI:EpsJ heterodimers. The EpsI:EpsJ dimer observed confirms a right-handed architecture of the pseudopilus, but, compared to a previous structure of the EpsI:EpsJ heterodimer, EpsI differs 6 degrees in orientation with respect to EpsJ; one loop of EpsJ is shifted by approximately 5A due to interactions with the nanobody; and a second loop of EpsJ underwent a major change of 17A without contacts with the nanobody. Clearly, nanobodies accelerate dramatically the crystallization of recalcitrant protein complexes and can reveal conformational flexibility not observed before. | lld:pubmed |
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| pubmed-article:19118632 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19118632 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19118632 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:19118632 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19118632 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19118632 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:19118632 | pubmed:issn | 1095-8657 | lld:pubmed |
| pubmed-article:19118632 | pubmed:author | pubmed-author:HolWim G JWG | lld:pubmed |
| pubmed-article:19118632 | pubmed:author | pubmed-author:KorotkovKonst... | lld:pubmed |
| pubmed-article:19118632 | pubmed:author | pubmed-author:SteyaertJanJ | lld:pubmed |
| pubmed-article:19118632 | pubmed:author | pubmed-author:PardonElsE | lld:pubmed |
| pubmed-article:19118632 | pubmed:author | pubmed-author:LamAnita YAY | lld:pubmed |
| pubmed-article:19118632 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:19118632 | pubmed:volume | 166 | lld:pubmed |
| pubmed-article:19118632 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19118632 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19118632 | pubmed:pagination | 8-15 | lld:pubmed |
| pubmed-article:19118632 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
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| pubmed-article:19118632 | pubmed:meshHeading | pubmed-meshheading:19118632... | lld:pubmed |
| pubmed-article:19118632 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19118632 | pubmed:articleTitle | Nanobody-aided structure determination of the EpsI:EpsJ pseudopilin heterodimer from Vibrio vulnificus. | lld:pubmed |
| pubmed-article:19118632 | pubmed:affiliation | Department of Biochemistry, Biomolecular Structure Center, University of Washington, 1959 Pacific Ave. NE, HSC K-428, Seattle, WA 98195, USA. | lld:pubmed |
| pubmed-article:19118632 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19118632 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:19118632 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:19118632 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
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