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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2009-1-16
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pubmed:abstractText |
The majority of alveolar RMSs have t(2;13)(q35;q14) or (1:13)(p36;q14),which generate PAX3/7 -FKHR fusion genes. Here, the authors detected the PAX3/7-FKHR fusion transcripts in 17 formalin-fixed, paraffin-embedded RMSs and 26 other SRCTs using one-step RT-PCR. PAX3 -FKHR and PAX7-FKHR transcripts were positive in 4/8 and 2/8 cases of ARMS, respectively. 9 ERMSs and 26 other SRCTs were negative for PAX3/7-FKHR. In addition, AChR-gamma and AChR-alpha mRNA were detected by semiquantitative duplex PCR in above cases and 3 normal muscles. 17 RMSs were found to have overexpression of AChR-gamma, with an AChR-gamma/-alpha ratio of > or =1; 3 cases of normal muscle had very weak AChR-gamma expression, with an AChR- gamma/-alpha ratio of <1. AChR-gamma transcripts were not detectable in all 26 other SCRTs. The results demonstrated that detection of PAX3/7-FKHR fusion gene by one-step RT-PCR is useful in the diagnosis of RMS and that AChR-gamma is overexpressed in Chinese RMS patients.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CHRNG protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/FOXO1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Fixatives,
http://linkedlifedata.com/resource/pubmed/chemical/Forkhead Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Formaldehyde,
http://linkedlifedata.com/resource/pubmed/chemical/PAX3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/PAX7 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/PAX7 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Paired Box Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cholinergic,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Nicotinic,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Markers, Biological
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1066-8969
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
17
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6-15
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pubmed:dateRevised |
2011-7-15
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pubmed:meshHeading |
pubmed-meshheading:18988640-Adolescent,
pubmed-meshheading:18988640-Adult,
pubmed-meshheading:18988640-Aged,
pubmed-meshheading:18988640-Case-Control Studies,
pubmed-meshheading:18988640-Child,
pubmed-meshheading:18988640-Child, Preschool,
pubmed-meshheading:18988640-China,
pubmed-meshheading:18988640-Female,
pubmed-meshheading:18988640-Fixatives,
pubmed-meshheading:18988640-Forkhead Transcription Factors,
pubmed-meshheading:18988640-Formaldehyde,
pubmed-meshheading:18988640-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:18988640-Gene Fusion,
pubmed-meshheading:18988640-Humans,
pubmed-meshheading:18988640-Infant,
pubmed-meshheading:18988640-Male,
pubmed-meshheading:18988640-Middle Aged,
pubmed-meshheading:18988640-PAX7 Transcription Factor,
pubmed-meshheading:18988640-Paired Box Transcription Factors,
pubmed-meshheading:18988640-RNA, Messenger,
pubmed-meshheading:18988640-Receptors, Cholinergic,
pubmed-meshheading:18988640-Receptors, Nicotinic,
pubmed-meshheading:18988640-Rhabdomyosarcoma, Alveolar,
pubmed-meshheading:18988640-Soft Tissue Neoplasms,
pubmed-meshheading:18988640-Tumor Markers, Biological,
pubmed-meshheading:18988640-Young Adult
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pubmed:year |
2009
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pubmed:articleTitle |
PAX--FKHR fusion genes and AChR-gamma in Chinese patients with rhabdomyosarcoma: diagnosis using formalin-fixed archival tissues.
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pubmed:affiliation |
Department of Pathology and Laboratory of Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine, Shihezi, Xinjiang, China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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