Linked Life Data

18979527

Source:http://linkedlifedata.com/resource/pubmed/id/18979527

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2009-2-9
pubmed:abstractText
Hydrophilic interaction chromatography (HILIC) with mass spectrometry is a versatile technique for structural glycomics. Glycans are retained by hydrogen bonding, ionic interactions, and dipole-dipole interactions. Glycopeptides as well as glycans with various modifications and reducing-end labels can be efficiently separated, which often results in the resolution of isobaric species. Chromatography is usually performed with solvent mixtures of organic modifier (often acetonitrile) and volatile (acidic) buffer which are suitable for online-electrospray ionization-mass spectrometry. When performed at the nano-scale, this results in a detection limit for oligosaccharides of approximately 1 femtomol. Alternatively, glycans may be analyzed by offline-MALDI-MS(/MS) in both negative-ion mode and positive-ion mode, which allows the registration of informative fragment ion spectra from deprotonated species and sodium adducts, respectively. (c) 2009 Wiley Periodicals, Inc., Mass Spec Rev 28:192-206, 2009.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:issn
1098-2787
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
28
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
192-206
pubmed:meshHeading
pubmed:articleTitle
Structural glycomics using hydrophilic interaction chromatography (HILIC) with mass spectrometry.
pubmed:affiliation
Leiden University Medical Center, Biomolecular Mass Spectrometry Unit, Department of Parasitology, P.O. Box 9600, 2300 RC Leiden, The Netherlands. m.wuhrer@lumc.nl
pubmed:publicationType
Journal Article, Review